Department of Biochemistry

Faculty of Medicine

Research


Prof. J.G.S. Ranasinghe
Research Interests
  • Effects of probiotics, fermented milk products  on serum parameters 
  • Investigation of iron balane in Thalasemic patiens treated with single and combination of drugs 
  • Study of heavy metal and ceruloplasmin in health problems. 
  • Effects of supplementation of Se, prebiotics, probiotics and nucleotides on  serum parameters 
  • A study on genetic variation, toxicity, morphology of giant honey bees of Sri Lanka. 
  • Comparison of toxic effects of Bungarus species in Sri Lanka 
  • Characterization of venom of Sri Lankan Snakes 
  • Effects of endogenous medicine on toxic effects of cobra venom.
Oral and Poster Presentation( with abstract) 2010
  • M Suziki, T Ithoh, BM Anuruddhe,, IK Badaranayake, JGSRanasinghe, S Athauda, Molecular diversity in venom proteins of the Russell’s viper (Daboia russellii russellii) and the Indian cobra (Naja naja) in Sri Lanka.  Biomedical research, 31 (1)71-81 2010
  • KH.K.T.Niroshini, R.P.V.J.Rajapakse, JGS.Ranasinghe, R.Kulasekera, K.B.A.T.Bandara, and N.A.N.D.Perera Study of antigenic salivary gland proteins of mosquitoes and detection of antibodies against them in rabbit  and human.Peradeniya university research sessions 2010. 
  • B.M.A.I.K.Bandaranayake, J.G.S.RanasinghePurification of Russell’s viper phospholipase A2 (RV-PLA2): Production and preliminary characterization of antibodies against RV-PLA2.Peradeniya university research sessions 2010. 
  • V.P. Gunaratne, A.G.G.Wijeratne, S.B.P.Athauda, R.M.P.Ratnayake, J.G.S.Ranasinghe and D.P.Nanayakara. Histopathological effects of crude venom and purified Phospholipase A(2) from Russell’s Viper (Daboia Russell II) in mice: A preliminary study. Peradeniya university research sessions 2010.
Oral and Poster Presentation( with abstract) 2011
  • VG Pinto, JGS Ranasinghe, JMN Priyadarshana, U Amarasinghe, GB Thenuwra, JMPVB Jayasinghe, WMSK Weerabahu, SU Dunukearachchi Can the spinal spread be changed with blood or CSF sodium concentrations?The college of anaesthesiologists of Sri Lanka, 27 th annual scientific sessions 2011
Journal Publication 2012
  • J.G.S. Ranasinghe. Effect of Mannan Oligosaccharides on Some Nutritional and Biochemical Parameters of Mice. Indian J. Anim. Nutr. 2012.29 (2) : 204-208
Journal Publications 2013 - International
  • Niroshini, S.K.H.K., Rajapakshe, R.P.V.J., Ranasinghe, J.G.S., Detection of antigenic proteins in salivary glands of female armigeres subalbatus mosquito. International Journal of Biological & Pharmaceutical Research (2013)     
  • Ranasinghe, J.G.S., Silva, S.S.P., Herath, N., Changes of serum lipids and proteins during probiotics feedings and its exposure.International Journal of Scientific and Research publications, 2013 Jan. 3(1): 1-5.         
Dr. H.K.I. Perera
Research interests
  • Antibiotic effects of medicinal plants
  • Acid Proteinases
  • Proteinase inhibitors
Oral and Poster Presentation( with abstract) 2010
  • H.K.I. Perera, D. Dissanayake (2010). Analysis of acid proteinase activity in early and late follicles of the ovary. Annual sessions of Kandy society of Medicine
  • T.N. Sudusinghe, D.D.K. Abeyaratne, W.A.N.T. Wickramarachchi, M.G.P.B.Maduranga, G.C.K. Amiyangoda, T.Choden, S.Sharmila, S.M.D. Sooriyarachchi,       D.M.S. Chalithanga, H.K.I. Perera, S. Agampodi. (2010). Association between     febrile convulsions and low birth weight of children and knowledge, attitudes and practices about febrile convulsions among their parents. Annual sessions of Kandy society of Medicine (This paper has received “ a commendable paper” award at the  32nd academic Annual sessions of Kandy society of Medicine- Presenter- T.N. Sudusinghe, Corresponding author- H.K.I. Perera).
  • H.K.I. Perera, Senarath B.P. Athauda and T. Takahashi (2010). The variation of acid proteinase activity at different stages of the follicular and luteal phases of the porcine ovarian cycle. Proceedings of Annual sessions of Peradeniya University Research Sessions15-1: 380-382.
  • W.I.T. Fernando, H. K. I. Perera , S.B. P. Athauda , N. S. Kumar, U. L. B. Jayasinghe and R Sivakanesan (2010). In-vitro screening of spices for lipase inhibitory activity and antioxidant activity. Annual sessions of Peradeniya University Research Sessions 15-1: 383-385.
  • A. Manamperi, H.K.I. Perera, H. Inoue and S.B.P.  Athauda (2010). Screening of some medicinal plant extracts for antiparasitic activity using Caenorhabditis elegans Annual sessions of Peradeniya University Research Sessions. 15-1: 386-388.
Oral and Poster Presentation( with abstract) 2011
  • H.K.I. Perera, W.I.T. Fernando and R. Sivakanesan. (2011). Sri Lankan studies validating antidiabetic potential of medicinal plants.International symposium on natural products and their applications in health and agriculture. p 67. 
  • W.I.T. Fernando, H. K. I. Perera, S.B. P. Athauda, N. S. Kumar, U. L. B. Jayasinghe and R Sivakanesan (2011). Lipase & amylase inhibitory actiivty of spices. International symposium on natural products and their applications in health and agriculture. p 69.
  • W.I.T. Fernando, H. K. I. Perera, S.B. P. Athauda, N. S. Kumar, U. L. B. Jayasinghe and R Sivakanesan (2011).            Heat stability of the amylase and lipase inhibitors in methanol extracts of some spices.  Proceedings of Annual sessions of the Sri Lanka Association for the Advancement of Science. p 6.
  • U.V.R. Senaratne, H.K.I. Perera, A. Manamperi and S.B.P. Athauda (2011). Partial purification of anthelmintic compounds from Solanum indicum. Annual sessions of Peradeniya University Research Sessions.16: 78 
  • W.I.T. Fernando, B.M.S.  Amarajeewa and H. K. I. Perera (2011).  Antihyperlipidaemic potential of polyherbal formula “lekhaneeya dashakaya”. Annual sessions of Peradeniya University Research Sessions.16: 79
  • D.B.G.M. Ekanayake, H.K.I. Perera and S.B.P. Athauda (2011). Analysis of blood parameters and RT-PCR results in dengue suspected patients from Kandy and Padaviya. Annual sessions of Peradeniya University Research Sessions.16: 80
Oral and Poster Presentation( with abstract) 2012 
  • U.V.R. Senaratne, H.K.I. Perera, A. Manamperi and S.B.P. Athauda (2012). Potential anthelminticcompounds from Solanum indicumfruit. International conference on chemical sciences
  • B.D.S. Jayawardana, H.K.I Perera and S. Rajapakse (2012). Protease inhibitory activity of some medicinal plants in Sri Lanka. Submitted for Annual sessions of Peradeniya University Research Sessions.
  • H.K.I. Perera, P.H.P. Fernando and S.B.P. Athauda (2012). Analysis of porcine ovarian extracts for the presence of multiple aspartic proteinases. Submitted for Annual sessions of Peradeniya University Research Sessions.
  • B. Sivasothy, H.K.I Perera and W.I.T.Fernando (2012). Inhibitory effect on pancreatic amylase by medicinal plants collected from Jaffna. Submitted for Annual sessions of Peradeniya University Research Sessions.
  • D.C.R Wijetunge, H.K.I Perera and A.M.P.S.T.M Bandara (2012). Gel Electrophoresis - A Tool to Analyze Protein Glycation and Inhibition. Submitted for Annual sessions of Peradeniya University Research Sessions.
Journal Publications 2013 - International
  • Ekanayake, D.B.G.M., Perera, H.K.I., Ellepola, A.N.B., Athauda, S.B.P., Analysis of blood parameters & RT-PCR results in dengue suspected patients from Sri Lanka.  International Journal of Research in Medical and Health Sciences, 2013 Sep. 3(1): 21-7.
Dr Kalana Maduwage
Research Interest
  • Toxinology, Venomous snake systematics, Analytical Biochemistry, Biomedical inventions
Oral and Poster Presentation (with abstract) 2011
  • 2011. The in vitro toxicity of venoms from South Asian Hump-nosed pit vipers (Viperidae: Hypnale), 10th Asia Pacific Association of Medical Toxicology Conference, Penang, Malaysia.
  • 2011. Comparative study of Merrem’s hump-nosed pit viper (Hypnale hypnale) bites between two climatic zones (wet vs dry zone) in Sri Lanka. 10th Asia Pacific Association of Medical Toxicology Conference, Penang, Malaysia.
  • 2011. Hump-nosed pit viper (Genus: Hypnale) envenoming in Sri Lanka: A study of epidemiology and clinical profiles across species. 10th Asia Pacific Association of Medical Toxicology Conference, Penang, Malaysia.
  • 2011. Pushpakumara J.P.H.G., Priyashantha A.G. Prasad, M.K. Prasad, M.K.M. Prasanna, A.A. Pandian, R. Ranaraja, R.M.R.D. Ranasinha, Y & Ranaweera, U.P.N.P. Effects of environmental temperature on visual and auditory reaction time in healthy young adults. The Kandy Society of Medicine, 33rd Annual Academic Sessions. 36-37.
  • 2011. Medagedara, S.C. Kularatne, S.A.M. Maduwage, K. Sivansuthan, S & de Silva, A. Epidemiology of snake bites in Jaffna Peninsula: A hospital based study. The Kandy Society of Medicine, 33rd Annual Academic Sessions. 130 (abstract).
  • 2011. Kularatne S.A.M, Maduwage K, Sivansuthan S, Ratnayake HMCD, Paranagama R, Mendis S, Medegadara SC, Ratnayake RIS, 2011, A descriptive study of offending species and epidemiology of snake bites of two areas in dry zone of Sri Lanka, Anuradhapura and Jaffna. Proceedings of the Peradeniya University Research Sessions. Sri Lanka.
Oral and Poster Presentation (with abstract) 2012
  • 2012. Clotting studies and factor deficiencies of Hump-nosed pit viper (Hypnale hypnale) envenoming in Sri Lanka, 11th Asia Pacific Association of Medical Toxicology Conference, Hong-Kong.
  • 2012. Efficacy of two Indian polyvalent snake antivenoms against Sri Lankan Russell’s viper (Daboia russelii) and Saw scaled viper (Echis carinatus) venom. 11th Asia Pacific Association of Medical Toxicology Conference, Hong-Kong.
Journal Publication 2010
Silva, A, K. Maduwage & R. Pethiyagoda. A review of the genus Rasbora in Sri Lanka, with description of two new species (Teleostei: Cyprinidae). Ichthyological Exploration of Freshwaters 21: 27-50.
Journal Publication 2011
  • 2011. Kularatne, S. A. M., Sivansuthan, S, Medagedara, S. C., Maduwage,K., de  Silva, A. Revisiting saw-scaled viper (Echis carinatus) bites in the Jaffna Peninsula of Sri Lanka: ecology, epidemiology and clinical manifestations. Transactions of the Royal Society of Tropical Medicine and Hygiene 105: 591-597. 
  • 2011. Maduwage, K., Hodgson, W. C, Konstantakopoulos, N., O’Leary, M. A., Gawarammana I., Isbister, G. K. The in vitro toxicity of venoms from South Asian Hump-nosed pit vipers (Viperidae: Hypnale). Journal of Venom Research 2: 17-23.
  • 2011. Gower, D., Maduwage K. Two new species of Rhinophis Hemprich (Serpentes: Uropeltidae) from Sri Lanka. Zootaxa. 2881: 51-68.
  • 2011. Kularatne, K., Sudhara, B., Maduwage, K., Naser, K., Kumarasiri, R., Kularatne, S., Parallels between Russell’s viper (Daboia russelii) and hump-nosed viper (Hypnale species) bites in the central hills of Sri Lanka amidst the heavy burden of unidentified snake bites. Asian Pacific journal of Tropical Medicine. 2011: 546-567.
  • 2011. Maduwage K., Kularatne K, Wazil A, Gawarammana I. Coagulopathy, acute kidney injury and death following Hypnale zaraenvenoming – The first case report from Sri Lanka. Toxicon, 58 (2011): 641–643
Journal Publication 2012 
  • 2012. Anjana Silva, Panduka Gunawardena, Danister Weilgama, Kalana Maduwage, Indika GawarammanaComparative in-vivo toxicity of venoms from South Asian hump-nosed pit vipers (Viperidae: Crotalinae: Hypnale)BMC Research Notes 2012, 5:471 .
  • 2012. Pethiyagoda, R., Meegaskumbura, M., Maduwage, K. A synopsis of the Indian and Sri Lankan fishes referred to Puntius Hamilton (Teleostei: Cyprinidae: Cyprininae). Ichthyological Exploration of Freshwaters 23 (1): 69–95.
Dr SBP Athauda
Oral and Poster Presentation( with abstract) 2010
  • Silva HNM. Vasantha Krishnan RKBS, Siribaddana AD, Peiris MD & Athauda SBP (2010) Molecular Diagnosis and species identification of TB in Kandy and Matale districts. University of Peradeniya Research Sessions 2010. 15:77-79
  • A.Manamperi, H.K.I.Perera, H.Inoue and S.B.P.Athauda(2010). Screening of some medicinal plant extracts for antiparasitic activity using Caenorhabditis elegans.Accepted for Annual sessions of Peradeniya University Research session 2010.
  • Bandara NASDJ, Rajapakse S and Athauda SBP (2010) Partial Purification and characterization of a trypsin inhibitor from the seed extract of Tamarindus indica. University of Peradeniya Research session 2010.
  • Bandara AMTK, Rajapaksha S, Inoue H and Athauda SBP (2010) Isolation of thermophilic bacteria and its deoxyribonucleases from hot springs at Trincomalee. University of Peradeniya Research sessions 2010. 15:391-393
  • V.P. Gunaratne, A.G.G.Wijeratne, S.B.P.Athauda, R.M.P.Ratnayake, J.G.S.Ranasinghe and D.P.Nanayakara. Histopathological effects of crude venom and purified Phospholipase A(2) from Russell’s Viper (Daboia Russell II) in mice: A preliminary study. Peradeniya university research sessions 2010.
Oral and Poster Presentation( with abstract) 2011
  • Senaratne UVR, Perera HKI, Manamperi A, Athauda SBP, 2011, Partial putrifaction of antihelmentic compounds from Solanum indicum.PURSE 2011 
  • Ekanayake DBGM, Perera HKI, Athauda SBP, 2011, Analysis of blood parameters and RT-PCR results in dengue suspected patients from Kandy and padaviya.PURSE 2011
Dr.WIT Fernando
Research interest
  • Natural Product Chemistry
  • Herbal medicine
Oral and Poster Presentation( with abstract) 2010 
  • W.I.T.Fernando, H.K.I.Perera, S.B.P.Athauda, N.S.Kumar, U.L.B.Jayasinghe and R.Sivakanesan. In-vitro screening of species for lipase inhibitory activity and antioxidant activity. Proceedings of Peradeniya University Research Sessions(PURSE) 2010 15:383-385
Oral and Poster Presentation( with abstract) 2011
  • Fernando WIT, Amarajeewa BMS, , Perera HKI, 2011, In- vitro inhibition of pancreatic lipase by the polyherbal formula ‘Lekhaneeya Dashakaya’PURSE 2011
  • WIT Fernando, HKI Perera, SBP Athauda, NS Kumar, ULB Jayasinghe and R.Sivakanesan(2011). Lipase & Amylase Inhibitory Activity of Species. IFS – AFASSA International Symposium On Natural Products And Their Applications In Health And Agriculture October 3-8:69
  • Perera HKI., Fernando, WIT, SIVAKANESAN, R. (2011). Sri Lankan studies validating antidiabetic potential of medicinal plants.Proceedings of the International Symposium on Natural Products and their Applications in Health and Agriculture, p 67 
  • Fernando, WIT., Perera HKI., Athauda SBP., Kumar, NS., Jayasinghe, ULB, SIVAKANESAN, R. (2011). Heat stability of the amylase and lipase inhibitors in methanol extracts of some spices. Proceedings of the 67th Annual Sessions, Sri Lanka Association for the Advancement of Science, p 6 
  • Fernando WIT, Amarajeewa BMS, , Perera HKI, 2011, In- vitro inhibition of pancreatic lipase by the polyherbal formula ‘Lekhaneeya Dashakaya’. Proceedings Of Peradeniya University Research Sessions(PURSE) 2011. 16:79
Dr.P.H.P.Fernando
Research interest
  • Lipid metabolism
  • Blood sugar homeostasis
  • Natural products research
Oral and Poster Presentation( with abstract) 2011
  • Lakshika Gunasekara, Fernando, PHP, Sivakanesan, R. (2011). A preliminary study on the hypoglycaemic effect of Averrhoa carambola (star fruit) in rats. Proceedings of the Peradeniya University Research Sessions (PURSE), Volume 16, p 83
Dr. C.N.R.A. Alles
Journal Publications 2013 - International
  • Khan, A.A.M., Alles, N., Soysa, N.S., Mamun, M.A.A., Nagano, K., Mikami, R., Furuya, Y., Yasuda, H., Ohya, K., Aoki, K., The local administration of TNF – a and RANKL antagonist peptide promotes BMP-2-induced bone formation. Journal of Oral Biosciences, 2013 Feb. 55(1): 47-54.      
  • Mamun, M.A.A., Khan, A.A.M., Alles, N., Matsui, M., Tabata, Y., Ohya, K., Aoki, K., Gelatin hydrogel carrier with the W9-peptide elicits synergistic effects on BMP–2 – induced bone regeneration. Journal of Oral Biosciences, 2013. 55(4): 217-23.  
  • Nakamura, H., Aoki, K., Masuda, W., Alles, N., Nagono, K., Fukushima, H., Osawa, K., Yasuda, H., Nakamura, I., Mikuni – Takagaki, Y., Ohya, K., Maki, K., Jimi, E., Disruption of NF-KB1 prevents bone loss caused by mechanical unloading. Journal of Bone and Mineral Research, 2013. 28(6):1457-67.